MICROBIOLAB
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MICROBIOLAB
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Laboratory-Equipments-Microbiology-Lab-Renae-Notes.pdf Flashcards
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A common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing open at the top and closed at the bottom
Test tube
Containers used in laboratories, available in various sizes with rough volume estimates
Beakers
Often used as reaction vessels, particularly in titrations, with volume markings that may not be accurate
Erlenmeyer flask
Used to measure and store solutions with high accuracy, possessing a marking indicating liquid volume equal to the volume written on the flask
Volumetric flask
Used to transfer liquids with a moderate degree of accuracy
Graduated cylinder
Used for transferring liquids with a fixed volume and known quantity to a high degree of accuracy
Pipette
Calibrated in the factory to release a desired quantity of liquid
Graduated pipette
Made of plastic and useful for transferring liquids dropwise
Disposable pipette
Devices used in analytical chemistry for measuring liquid solution, heavily used in titration experiments
Burette
Scientific equipment used to hold and secure a burette on a stand for experiments
Burette clamp
A wide-top, narrow-bottom tube or pipe used for guiding liquid or powder into a small opening
Funnel
Piece of laboratory equipment used in filtration, traditionally made of porcelain with variations in glass and plastic
Buchner funnel
Standard technique for separating solid-liquid mixtures with the goal of retaining the solid, aided by suction beneath the funnel
Vacuum filtration
Used to hold round laboratory glassware like beakers and flasks, typically made from stainless steel
Clamp
Brush used for cleaning test tubes, narrow-mouth glassware, and laboratory equipment
Test tube brush
Retain the solid in crystallization
Gravity filtration
Process aided by suction beneath the funnel
Crystallization
Used to hold round laboratory glassware
Clamp
Brush used for cleaning test tubes
Test tube brush
Used to hold test tubes in place
Test tube holder
Laboratory equipment used to hold multiple test tubes upright
Test tube stand
Source of heat in laboratories
Bunsen burner
Small dish shaped like a cylinder
Petri dish
Used to mix chemicals and liquids in laboratories
Glass rod
Instrument used to transfer small quantities of liquid
Graduated dropper
Type of tool used to grip and lift objects in laboratories
Tongs
Laboratory apparatus resembling a pair of scissors
Utility clamp
Three-legged platform used to support flasks and beakers
Tripod for Bunsen burner
Squeeze bottle with a nozzle used to rinse laboratory glassware
Wash bottle
Used for uniform distribution of flame heat in a heated body
Wire mesh gauze
Laboratory-Equipments-Microbiology-Lab-Renae-Notes.pdf Flashcards
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Magnetic Stirrer
A device widely used in laboratories that consists of a rotating magnet or a stationary electromagnet creating a rotating magnetic field, used for stirring or mixing solutions
Water Bath
Laboratory equipment made from a container filled with heated water, used to incubate samples at a constant temperature over a long period of time
Vacuum Pump
A device used to evacuate chemically aggressive gases and vapors
Digital Colorimeter
A light-sensitive device used for measuring the transmittance and absorbance of light passing through a liquid sample to determine color intensity or concentration
Digital Conductometer
An instrument that measures the electrical conductivity in a solution, with applications in research and engineering
Digital pH Meter
A scientific instrument that measures the hydrogen ion activity in water-based solutions, indicating acidity or alkalinity expressed as pH
Digital Potentiometer
An instrument for measuring voltage by comparing an unknown voltage with a known reference voltage, providing high precision in measurement
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Microscope-Microbiology-Lab-Renae-Notes.pdf Flashcards
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Contains lenses that contribute to total magnification power of 10x
Ocular eyepiece
Hollow tube that keeps the lenses of the ocular and objectives at a set distance
Body tube
Holds objectives
Nosepiece
Contain lenses that contribute to total magnification
Objectives
Supports body tube
Arm
Supports the entire microscope; keep hand back on base when carrying because lamp will be hot
Base
Traylike structure that supports specimen slide over the stage opening
Stage
Keeps specimen slide tight against the stage
Stage clips
Controls the amount of light that reaches your eye
Diaphragm
Provides light to create the image that you see
Light source
Larger knob that moves body tube, stage, or nosepiece for rough focus
Coarse adjustment
Smaller knob that moves the objectives slightly for fine focusing
Fine adjustment
What part you look through in a microscope
Ocular eyepiece
Part of the microscope that holds the objectives
Nosepiece
Part that keeps the lenses of the ocular and objectives at a set distance
Body tube
What is a microscope?
A laboratory equipment that is used to magnify objects that cannot normally be seen by the unaided eye.
What is the function of a microscope?
It magnifies objects producing an image in which the object appears larger.
What are the major types of microscopes?
1. Compound Microscope 2. Dissecting Microscope 3. Electron Microscope
When was the first compound microscope created?
1590 by two Dutch spectaclemakers, Hans and Zacharias Janssen.
Who discovered plant cells using a compound microscope?
Robert Hooke in 1670.
Who was the first to observe bacteria using a microscope with one lens?
Anton van Leeuwenhoek in 1675.
Where should you always store your microscope?
With the low power objective in place.
What is the total magnification of a microscope with an ocular objective of 10x and a high power objective of 50x?
500x total magnification
Where are the objectives of a microscope attached?
Nosepiece, which can be rotated to click the lenses into place.